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KMID : 0368019940170041411
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Journal of Soonchunhyang University
1994 Volume.17 No. 4 p.1411 ~ p.1426
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The Characteristics of Cultured Endothelial Cells Infected by Rickettsia typhi
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Abstract
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R. typhi, the etiologic agent of murine typhus, has known to cause fever, headache, maculopapular rash and vasculitis of small blood vessel. The skin rash and vasculitis are thought to be the results of endothelial cell injury from the infection
with
R.typhi in vivo.
R.typhi showed ability to cause cellular injury of cultured human endothelial cells. The endothelial cells used in this experiment were obtained by collagenase treatment of human umbilical cord veins. These cells were conformed to be endothelial
cell by
the presence of von Willebrand factor through the immunofluorescent antibody technique and Weibel-Palade bodies under the electron microscopy. Ultrastructural study revealed that there were no evidences of entry and/or escape from endothelial
cells
of
R.typhi in 24 hours through 48 hours after infection. On the contrary, numerous particles implicating R.typhi were observed in large vacuole within endothelial cells at 48 hours after inoculations.
Estimating the severe injury of endothelial cells by infection of R.typhi, the endothelial cells seems to be the primary target cells in human disease cause by R.typhi infection.
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